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KMID : 0811720020060050261
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Korean Journal of Physiology & Pharmacology
2002 Volume.6 No. 5 p.261 ~ p.267
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Interactions between Estradiol-17β-BSA and Calcitropic Hormones in Ca2+ Uptake in Renal Proximal Tubule Cells
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Ho Jae Han/Ho Jae Han
Yeun Hee Lee/Eun Ju Seo
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Abstract
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The aim of the present study was to investigate the interaction of estradiol?17β?bovine serum albumin (E2?BSA) and calcitropic hormones, such as parathyroid hormone, calcitonin, and vitamin D, in regulation of Ca2+ uptake in primary cultured renal proximal tubule cells. Statistically significant increase in Ca2+ uptake was found from 2 hours after (E2?BSA)(10?9M) treatment, while estradiol?17β(10?9M) did not affect. Treatment of the cells with (E2?BSA)(10?9M) together with parathyroid hormone (PTH) (10?8M), vitamin D (10?8M), or calcitonin (10?8M) significantly stimulated Ca2+ uptake by 32.50%, 29.30%, or 27.75%, respectively, compared with the control. However, calcitropic hormones did not exhibit any synergistic effect on the E2-BSA-induced stimulation. E2?BSA significantly increased cAMP generation and PKC activity. The stimulatory effect of cotreatment of E2?BSA and PTH or vitamin D was blocked by SQ22536 (an adenylate cyclase inhibitor) and staurosporine (a PKC inhibitor), but the effect of cotreatment of E2?BSA and calcitonin was not blocked. Furthermore, 8-Br-cAMP and TPA (an artificial PKC promoter) increased Ca2+ uptake by 25.51% and 16.47%, respectively, compared with the control. In conclusion, E2?BSA combined with calcitropic hormones regulated Ca2+ uptake partially via cAMP and PKC-dependent mechanisms in renal proximal tubule cells.
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KEYWORD
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E2-BSA, Ca2+ uptake, PTH, vitamin D, calcitonin, cAMP, PKC,
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